Minimum Inhibitory Concentration Determination of Bambusa vulgaris Leaves Extract Against Skin and Digestive Diseases Bacteria

Penulis

  • Kelly Zhiang Program Studi Farmasi Fakultas Kedokteran Universitas Tanjungpura
  • Rafika Sari Program Studi Farmasi Fakultas Kedokteran Universitas Tanjungpura
  • Pratiwi Apridamayanti Program Studi Farmasi Fakultas Kedokteran Universitas Tanjungpura

DOI:

https://doi.org/10.26874/kjif.v7i1.158

Abstrak

Infections that are commonly happened in developing countries such as Indonesia are skin infection that caused by Staphylococcus aureus and Staphylococcus epidermidis, and digestive infection that caused by Bacillus subtilis and Bacillus cereus. The ethanol extract of Bambusa vulgaris leaves contained metabolite compounds that potential to have antibacterial activity. This study aimed to determine MIC (Minimum Inhibitory Concentration) value of ethanol extract of Bambusa vulgaris leaves against those four bacteria. The research was conducted by Kirby-Bauer disc diffusion method using ethanol extract of Bambusa vulgaris leaves with concentration variations of 80 mg/mL, 40 mg/mL, 20 mg/mL, 10 mg/mL, 5 mg/mL, 2.5 mg/mL, and 1.25 mg/mL. The results showed that ethanol extract of Bambusa vulgaris leaves can inhibit the growth of all four bacteria with MIC value against Staphylococcus aureus and Bacillus subtilis is 20 mg/mL, with inhibition zone diameter of 7,33 ± 0,416 mm and 6,32 ± 0,057 mm respectively. While the MIC value against Staphylococcus epidermidis and Bacillus cereus is 10 mg/mL, with inhibitory zone diameter obtained respectively 7,33 ± 0,416 mm and 6,07 ± 0,029 mm. The conclusion of this research was that ethanol extract of Bambusa vulgaris leaves had inhibition zone with MIC value 20 mg/mL against Staphylococcus aureus and Bacillus subtilis, and 10 mg/mL against Staphylococcus epidermidis and Bacillus cereus.

Keywords: Ethanol extract of Bambusa vulgaris leaves, antibacterial, disc diffusion, MIC (Minimum Inhibitory Concentration)

Biografi Penulis

Rafika Sari, Program Studi Farmasi Fakultas Kedokteran Universitas Tanjungpura

Departemen Biologi Farmasi

Pratiwi Apridamayanti, Program Studi Farmasi Fakultas Kedokteran Universitas Tanjungpura

Departemen Kimia Farmasi

Referensi

Akbar, M. R. V., Budiarti, L. Y., & Edyson. (2016). Perbandingan Efektivitas Antibakteri antara Ekstrak Methanol Kulit Batang Kasturi dengan Ampisilin terhadap Staphylococcus aureus In Vitro. Berkala Kedokteran, 12(1), 1–9.

Ambika, K., & Rajagopal, B. (2017). In Vitro Antimicrobial and Antiproliferative Activity of Bambusa vulgaris. Human Journals, 9(1), 10–22.

Annafiatuzakiah. (2017). Studi Etnofarmakologi Toksisitas Akut dan Analgesik Ekstrak Etanol Daun Bambu (Bambusa vulgaris) Tanaman Endemik Kalimantan Barat. Universitas Tanjungpura.

Astutiningrum, T. (2016). Uji Aktivitas Antibakteri Ekstrak Daun Kenikir (Cosmos caudatus Kunth.) terhadap Pertumbuhan Bakteri Staphylococcus aureus secara In-Vitro. Universitas Sanata Dharma.

Ayyagari, A., Gandhi, S., & Sushma, G. (2009). Detection of Antimicrobial Resistance in Common Gram Negative and Gram Positive Bacteria Encountered in Infectious Diseases – An Update. ICMR Bulletin, 39(1–3), 1–20. https://doi.org/ISSN 0377-4910

Black, J. M., & Jacobs, E. M. (1997). Medical Surgical Nursing: Clinical Management for Continuity Care (5th ed.). Philadelphia: Saunders.

Brooks, G. F., Carroll, K. C., Butel, J. S., Morse, S. A., & Mietzner, T. A. (2007). Mikrobiologi Kedokteran Jawetz, Melnick, & Adelberg. (H. Hertanto, C. Rachman, A. Dimanti, & A. Diani, Eds.) (23rd ed.). Jakarta: EGC.

Depkes RI. (2013). Riset Kesehatan Dasar. Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementrian Kesehatan RI.

Hannif, Mulyani, N. S., & Kuscithawati, S. (2011). Faktor Risiko Diare Akut pada Balita. Berita Kedokteran Masyarakat, 27(1), 10–17.

Mayanti, T., Julaeha, E., & Putri A, Y. (2016). Isolasi dan Karakterisasi Senyawa Antibakteri dari Fraksi Etil Asetat Kulit Batang Lansium domesticum Corr. Cv Kokossan.

Meindl, P., & Chopra, S. (2007). Supply Chain Management: Strategy, Planning and Operasion (3rd ed.). New Jersey: Pearson Prentice Hall.

Naidu, A. S., & Clemens, R. (n.d.). Natural Food Antimicrobial System: Probiotics. New York: CRC Press.

Nugerahdita N. (2009). Prevalensi Penyakit Kulit dan Pengobatannya pada Beberapa RW di Kelurahan Petamburan Jakarta Pusat. Universitas Indonesia.

Nuria, M. C., Faizatun, A., & Sumantri. (2009). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jattopha curcas L) terhadap Bakteri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Salmonella typhi ATCC 1408. Mediagro, 5(2), 26–37.

Onajobi, I. B., Agbaje, A. B., & Alaba, S. A. (2015). In Vitro Antibacterial Assessment of Elaeis guineensis and Bambusa vulgaris Extracts Against Some Selected Pathogens. Nigerian Journal of Microbiology, 27(1), 2701–2708.

Owolabi, M. S., & Lajide, L. (2015). Preliminary Phytochemical Screening and Antimicrobial Activity of Crude Extracts of Bambusa vulgaris Schrad. Ex J.C. Wendl. (Poaceae) from Southwestern Nigeria. American Journal of Essential Oils and Natural Products, 3(1), 42–45.

Pangestuti, I. E., Sumardianto, & Amalia, U. (2017). Skrining Senyawa Fitokimia Rumput Laut Sargassum Sp. dan Aktivitasnya Sebagai Antibakteri Terhadap Staphylococcus aureus dan Eschericia coli. Indonesian Journal of Fisheries Science and Technology, 12(2), 98–102.

Rahmaningsih, S., Wilis, S., & Mulyana, A. (2012). Bakteri Patogen dari Perairan Pantai dan Kawasan Tambak di Kecamatan Jenu Kabupaten Tuban. Ekologia, 12(1), 1–5.

Robinson, T. (1995). The Organic Constituent of Higher Plants (4th ed.). Bandung: Institut Teknologi Bandung.

Sari, Y. D., Djannah, S. N., & Nurani, L. H. (2010). Uji Aktivitas Antibakteri Infusa Daun Sirsak (Annona muricata L.) secara In Vitro terhadap Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 35218 serta Profil Kromatografi Lapis Tipisnya. Kes Mas, 4(3), 218–238.

Septiani, Dewi, E. N., & Wijayanti, I. (2017). Aktivitas Antibakteri Ekstrak Lamun (Cymodocea rotundata) terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Saintek Perikanan, 13(1), 1–6.

Simaremare, E. S. (2014). Skrining Fitokimia Ekstrak Etanol Daun Gatal (Laportea decumana (Roxb.) Wedd). Pharmacy, 11(01), 98–107.

Sudewi, S., & Lolo, W. A. (2016). Kombinasi Ekstrak Buah Mengkudu (Morinda citrifolia L.) dan Daun Sirsak (Annona muricata L.) dalam Menghambat Bakteri Escherichia coli dan Staphylococcus aureus. Kartika J. Ilm. Far, 4(2), 36–42.

Sumadi, R. S. (2011). Identifikasi dan Uji Aktivitas Antibakteri Fraksi Teraktif Daun Sirih Merah (Piper crocatum Ruiz & Pau). Universitas Sebelas Maret.

Widyasanti, A., Hajar, S., & Rohdiana, D. (2015). Aktivitas Antibakteri Ekstrak Teh Putih terhadap Bakteri Gram Positif dan Negatif. Jurnal Penelitian Teh Dan Kina, 18(1), 55–60.

Wu, T., Zang, X., He, M., Pan, S., & Xu, X. (2013). Structure–Activity Relationship of Flavonoids on Their Anti-Escherichia coli Activity and Inhibition of DNA Gyrase. Journal of Agricultural and Food Chemistry, 61, 8185–8190. https://doi.org/10.1021/jf402222v

Zimbro, M. J., Power, D. A., Miller, S. M., Wilson, G. E., & Johnson, J. A. (2009). Difco & BBL Manual: Manual of Microbiological Culture Media (2nd ed.). Maryland: Becton, Dickinson and Company. https://doi.org/10.1002/1521-3773(20010316)40:6<9823::AID-ANIE9823>3.3.CO;2-C

Zubair, M., Bibi, Z., Rizwan, K., Rasool, N., Zahoor, A. F., & Riaz, M. (2013). In Vitro Antimicrobial and Haemolytic Studies of Bambusa arundinaceae Leaves. Journal of Applied Pharmaceutical Science, 3(4), 111–115. https://doi.org/10.7324/JAPS.2013.3420

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Diterbitkan

2019-12-28

Cara Mengutip

Zhiang, K., Sari, R., & Apridamayanti, P. (2019). Minimum Inhibitory Concentration Determination of Bambusa vulgaris Leaves Extract Against Skin and Digestive Diseases Bacteria. Kartika : Jurnal Ilmiah Farmasi, 7(1), 1–5. https://doi.org/10.26874/kjif.v7i1.158

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