EFEK ANTIBAKTERI KOMBINASI EKSTRAK KUNYIT (Curcuma domestica Val.) dan JAHE (Zingiber officinale Rosc.) TERHADAP Escherichia coli DENGAN METODE PITA KERTAS
Isi Artikel Utama
Escherichia coli adalah bakteri patogen yang menyebabkan penyakit diare. Pengobatan infeksi bakteri menggunakan antibiotik berpotensi menimbulkan resistensi. Tanaman herbal banyak digunakan sebagai pengobatan alternatif, termasuk rimpang kunyit dan jahe karena sifat antibakterinya. Penelitian ini bertujuan untuk mengetahui bagaimana pengaruh ekstrak kunyit dan jahe pada bakteri E. coli dengan metode pita kertas. Metode dilusi, difusi dan kombinasi pita kertas diguakan untuk menguji aktivitas antibakteri. Uji dilusi menunjukkan hasil MBC pada konsentrasi 25%. Uji difusi cakram untuk menentukan kombinasi terbaik dalam menghambat pertumbuhan E. coli dengan menggabungkan ekstrak dalam proporsi (1:1), (1:2), dan (2:1). Data statistik menunjukkan kombinasi terbaik dalam perbandingan (1:2). Efek kombinasi yang diuji dengan metode pita kertas menunjukkan bahwa kombinasi keduanya indiferen, yang berarti tidak saling mempengaruhi.
Kata kunci : Antibakteri, escherichia coli, jahe , kunyit, kombinasi, pita kertas.
Escherichia coli is a pathogenic bacterium that causes diarrheal diseases. Treatment of bacterial infections using antibiotics has the potential to cause resistance. Herbal plants are widely used as alternative medicines, including turmeric rhizomes and ginger which are known to have antibacterial activity. This study aims to determine the effect of the combination of turmeric extract and ginger on E. coli bacteria using the paper tape method. The antibacterial activity test was carried out by the method of dilution, diffusion and its combination effect with the paper tape method. The dilution test showed MBC results at a concentration of 25%. Disc method diffusion test to determine the best combination in inhibiting E. coli growth by combining extracts in ratios (1:1), (1:2), and (2:1). The statistical data show the best combination in comparison (1:2). The effect of the combination tested with the paper tape method showed that the combination of the two was indifferent, meaning that it did not affect each other.
Keywords: Antibacterial, escherichia coli, ginger, turmeric, combination, paper tape method.
Afriyana, R. and Junando, M., 2023. Potensial Ekstrak Herbal Kunyit (Curcuma Longa) Sebagai Anti Bakteri dan Anti Inflamasi Potency of Turmeric Herbal Extract (Curcuma Longa) As, 10, 128–132.
Amanda, R., Rijai, L., and Arifuddin, M., 2025. Isolation and Identification of Lactic Acid Bacteria from the Washing Water of Mayas Rice , a Traditional Mountain Rice from East Kalimantan Isolasi Dan Identifikasi Bakteri Asam Laktat Dari Air Cucian Beras Gunung (Beras Mayas) Khas Kalimantan Timur, (2), 96–104.
Azkiyah, S.Z., 2020. Pengaruh Uji Antibakteri Ekstrak Rimpang Jahe Terhadap Pertumbuhan Staphylococcus Aureus Dan Escherichia Coli Secara In Vitro Effect Of Antibacterial Test Of Ginger Ginger Extract On The Growth Of Staphylococcus Aureus And Escherichia Coli In Vitro, 1 (2), 71–80.
Balouiri, M., Sadiki, M., and Ibnsouda, S.K., 2016. Methods for in vitro evaluating antimicrobial activity : A review Journal of Pharmaceutical Analysis, 6 (2), 71–79.
Bobis, J., Nilsson, J., Göran, D., Larsson, J., and Flach, C., 2024. Journal of Global Antimicrobial Resistance Evaluation of culture conditions for sewage-based surveillance of antibiotic resistance in Klebsiella pneumoniae, 37, 122–128.
Cheesman, I., 2025. Isolasi , Identifikasi , dan Karakterisasi Mikroorganisme (Bakteri , Jamur , Gram dan Pewarnaan Spesifik mendegradasi komponen pangan seperti protein , lemak , dan karbohidrat menjadi senyawa, (September).
Eloff, J.N., 2019. Avoiding pitfalls in determining antimicrobial activity of plant extracts and publishing the results, 3, 1–8.
Irawan, A., Ulfah, M., Putra, T.A., and Hadi, I., 2022. Uji aktivitas antibakteri ekstrak aseton jahe merah (Zingiber officinale L.) terhadap bakteri escherichia coli antibacterial activity of extract aceton of red ginger (zingiber officinale l.) against bacterial gram negative , escherichia coli, 1.
Lu, A., Sari, R., and Apridamayanti, P., 2017. Penentuan Nilai FICI Kombinasi Ekstrak Etanol Kulit Daun Lidah Buaya ( Aloe vera ( L ) Burm . f ) dan Gentamisin Sulfat Terhadap Bakteri Escherichia coli FICI value determination of combination of Aloe vera ( L ) Burm . f coli Abstrak, 132–142.
Mao et al., 2019. Bioactive Compounds and Bioactivities of Ginger ( Zingiber o ffi cinale Roscoe), 1–21.
Mudau, K.L. and Ntobeng, L.R., 2025. Pathogenicity and virulence factors of Escherichia coli discovered using next generation sequencing technologies and proteomics, (October), 1–9.
Partridge, J.D. and Harshey, R.M., 2016. Swarming : Flexible Roaming Plans, 195 (5), 909–918.
Patel, R., 2015. Reviews MALDI-TOF MS for the Diagnosis of Infectious Diseases, 111.
Purbaya, S., Aisyah, L.S., and Arianti, W.E., 2018. Aktivitas Antibakteri Ekstrak Etil Asetat Jahe Merah (Zingiber officinale Roscoe var . sunti) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli, (1), 29–34.
Salma, A., Prajawanti, K.N., Aristia, B.F., and Nisyak, K., 2025. Potensi Jintan Hitam (Nigella sativa) sebagai Agen Antibakteri terhadap Bakteri Patogen Klinis.
Wulandari, Linda, A., 2021. Jurnal basicedu, 5 (5), 3034–3043.
Yanti, Y.N., Yanti, Y.N., and Mitika, S., 2017. Uji efektivitas antibakteri ekstrak etanol daun sambiloto (Andrographis paniculata Nees) terhadap bakteri Staphylococus aureus, 2 (1), 158–168.
