AKTIVITAS ANTIFUNGI SHAMPO DAN KRIM EKSTRAK ETANOLIK BATANG BROTOWALI TERHADAP Pityrosporum ovale DAN Trichophyton mentagrohytes
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ABSTRAK
Brotowali dengan kandungan alkaloid, flavonoid, dan terpenoid diketahui memiliki aktivitas antifungi. Tujuan penelitian ini adalah untuk mengetahui kadar hambat minimum ekstrak etanolik batang Brotowali dan aktivitas antifungi shampo dan krim ekstrak etanolik batang Brotowali terhadap P.ovale dan T.mentagrohytes. Batang Brotowali dimaserasi dengan etanol 70%, kemudian ekstrak etanolik batang brotowali diuji aktivitas antifungi untuk menentukan konsentrasi ekstrak yang akan digunakan dalam formulasi shampoo dan krim. Formula terbaik shampo diuji aktivitas terhadap P. ovale dengan metode cakram dan formula terbaik krim diuji aktivitas terhadap T. mentagrohytes menggunakan metode sumuran. Hasil penelitian menunjukkan KHM ekstrak etanolik batang Brotowali terhadap P. ovale adalah 900 ppm dan terhadap T.mentagrohytes 3,9 ppm. Formula shampo terbaik memiliki zona hambat antifungi kategori kuat 13,43+3,18 mm dengan persen daya hambat 69, 12% . Formula krim terbaik memiliki zona hambat antifungi kategori sedang 8,42+0,38 mm dengan persen daya hambat 62,74%.
Kata kunci : Brotowali, shampo, krim, P. ovale , T. mentagrohytes
ABSTRACT
Brotowali are known to have flavonoid, alcaloid, and terpenoid which have antifungal activity. This study aimed to determine the minimum inhibitory concentration ethanolic extract Brotowali stem and know the antifungal activity of shampoo and cream of ethanolic extract Brotowali stem to P.ovale and T.mentagrohytes. Brotowali stem macerated with 70% ethanol and test antifungal activity of ethanolic extract Brotowali stem to determine concentration used in shampoos and creams formula. The best shampoo formula tested antifungal activity against P.ovale and cream against T. mentagrohytes with compared method. The results showed MIC of ethanolic extract Brotowali stem against P.ovale was 900 ppm and against T. mentagrohytes was 3.9 ppm. The best shampoo formula has strong inhibitory zone antifungal category 13.43 + 3.18 mm and percent inhibition of 69, 12%. The best cream formula has currently inhibitory zone antifungal category was 8.42 + 0.38 mm and percent inhibition of 62.74%.
Keywords : Brotowali, shampoo, cream, P. ovale , T. mentagrohytes
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